3 Part+ Hematology Analyzer


Mispa count is a revolutionary analyser which will give you better cell counts than any other hematology analyser in the market. It used 50 micron aperture for RBC and PLT count which give enhance impedance pulse and make the detections of cells, especially platelets better. 50 mircon aperture are generally used in 5 part analyser and hence you can expect the result similar to 5 part. It double count the cells, after double counts Mispa count measure the variation if variation is low it will accept the results and give average value but if variation is high, average values will be displayed with Rejection Alarm.

Since it is better than any 3 part and have features of 5 part (50 micron aperture), it is not just any other 3 part anlayser. It is 3 Part + Hematology Analyser


 
  • Sample Volume: 10 µl
  • Throughput: 60 Samples per hour
  • Sample mode: Open vial
  • Type: Double chamber
  • 20 parameters with 3 histograms. WBC, LY%, LY#, MID%, MID#, GRAN%, GRAN#, RBC, HGB, HCT, MCV, MCH, MCHC, RDW-CV, RDW-SD, PLT, MPV, PDW, PCT, P-LCR
  • Touch screen
  • 5 USB ports, RS 232 port, LAN port, SUBD-9 pins male, RJ45
  • No of reagents: 3 no's. Diluent, Rinse and Lyse
  • Aperture size: 80 micron for WBC and 50 micron for RBC and PLT
  • Power: 100 to 240VAC, 50 to 60 Hz, 24V, 6.35A

1. WBC, RBC, PLT Counting through “Enhanced Electrical Impedance”
 
Enhanced Electrical Impedance is used to count the blood cells, blood cellss are classified based on their sizes.  When a blood cell in blood diluents mixture passes through an aperture, it cause change in electrical resistance. In this principal this change in resistance is measured to count and differentiate WBCs.  An electrode is sub merged in the liquid on both sides of the aperture to create an electrical pathway. As each particle passes through the aperture, a transitory change in the resistance between the electrodes is produced. This change produces a measurable electrical pulse. The numbers of cells are calculated by calculating the number of pulse generated. This pulse is dependent on the volume of cell, bigger the volume bigger is the pulse or vice versa.  Pulse is processed and only those pulse which are falling in certain ranges are taken for calculation of WBCs, RBCs and PLTs, these ranges are called as thresholds. If a pulse fall under these thresholds then only they are considered as particular type cells
 
  • Hemoglobin Measurement:
     
HGB is determined by the colorimetric method. The WBC/HGB dilution is delivered to the WBC bath where it is bubble mixed with a certain amount of lyse, which converts hemoglobin to a hemoglobin complex that is measurable at 525 nm. An LED is mounted on one side of the bath and emits a beam of light, which passes through the sample and a 525nm filter, and then is measured by a photo-sensor that is mounted on the opposite side. The signal is then amplified and the voltage is measured and compared to the blank reference reading(readings taken when there is only diluent in the bath). The HGB is calculated per the
following equation and expressed in g/L.

HGB(g/L) = Constant×Log 10 (Blank Photocurrent/Sample Photocurrent)
 

  •  Enhanced Electrical Impedance for Blood cells count
  •  Colorimetry for Hb by cyanide free method

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